For the past few years there has been a phenomenal rise in the cases of multi-drug resistance in microbes prompting the researchers to look into the newer anti-microbial agents. The goal of this study is to use an agar well test to evaluate the antibacterial activity of Abutilon indicum floral extract against Staphylococcus aureus and Escherichia coli. The flower extract of the ethanol (10µg/ml) was found to be highly active against Escherichia coli and Staphylococcus aureus as compared to the chloroform extract (10 µg/ml) towards the same bacterial species. The aqueous extract & DMSO had minimal efficacy towards all the 2 bacterial species studied. The known antibiotic, Chloramphenicol (10 µg/ml) however showed excellent activity against all 2 bacterial species. The plant is already well known in Ayurveda & as the plant has shown efficacy against 2 bacterial species, there is hope that many more anti-microbial agents would be discovered from this plant in the near future.